tuj  (Santa Cruz Biotechnology)

Journal: Bioactive Materials

Article Title: A cell motility-based selective hydrogel enables rapid generation of nerve-repairing blood clots

doi: 10.1016/j.bioactmat.2026.05.015

Figure Lengend Snippet: Effect and mechanism of the nano-gelatin on NSCs . (A) A diagram of the layered composite structure of the nano-gelatin after hemostasis. (B) Representative SEM images showing the platelet-derived extracellular vesicles in the nano-gelatin after hemostasis. (C) ALB contents in the cryogels following hemostasis (N = 4). (D) NGF contents in the cryogels (N = 3). (E) SDF-1 contents in the cryogels (N = 3). (F) Representative images showing NSCs migrating through the Transwell membrane into the plate with cryogels. (G) Quantification of the NSC numbers that migrated through the Transwell membrane into the plate (N = 4). (H) Representative images of the live/dead staining showing the survival and morphology of NSCs on the cryogels. (I) Cytotoxicity of the cryogels on NSCs by CCK-8 assay. (J) Representative images of immunostaining against F-actin, paxillin, and vinculin for cells encapsulated in the nano-gelatin and the GelMA hydrogel. (K) Representative images of immunostaining against Tuj-1 and GFAP. (L) Volcano plot analyzing DEGs between the nano-gelatin group and the control group. (M) The enriched GO pathways. (N) The enriched KEGG pathways. (O) The heatmaps of DEGs associated with Focal adhesion. (P) Schematic diagram of the potential mechanism by which the nano-gelatin regulates NSC migration and differentiation to promote nerve repair. Statistical analysis was performed using one-way ANOVA followed by Tukey's multiple comparisons test.

Article Snippet: Then, the samples were fixed and stained with astrocyte marker GFAP (1:500, CST, Rabbit mAb #80788) and neuron marker Tuj-1 (1:200, HUABIO, SP06-00) to assess differentiation.

Techniques: Derivative Assay, Membrane, Staining, CCK-8 Assay, Immunostaining, Control, Migration

Journal: bioRxiv

Article Title: Patient-Derived PSEN1 Cerebral Organoids Revealed Parallel Development of Amyloid-β Accumulation and Network Dysfunction

doi: 10.64898/2026.04.02.707990

Figure Lengend Snippet: A: Representative immunofluorescence image of a wild-type (WT) cerebral organoid section showing well-organised neuroepithelial zones and expression of key neuronal markers, including SOX2, PAX6, NeuN, CTIP2, MAP2, and TUJ, confirming neural differentiation and cortical identity at differentiation day 90 (DD90). Scale bar = 200 µm. B: Experimental design: cerebral organoids were generated from WT and AD iPSC lines and harvested at differentiation days 60, 90, and 130 (DD60, DD90, and DD130) for molecular, histological, and biochemical analyses. C: qPCR analysis of selected neural stem cell, neuronal, and glial markers in DD60-DD130 organoids. Results are normalised to mRNA expression in WT DD60. Each dot represents a pooled sample of 5–8 organoids (n = 2-4 independent experiments) and the lines represent mean ± SEM. D: Representative immunofluorescence image showing amyloid-β (Aβ) accumulation in AD organoid section at day 90 (DD90) using the D54D2 antibody. Scale bar = 200 µm. E: Quantification of intracellular Aβ signal intensity in WT and AD organoids across all timepoints, revealing progressive accumulation in the AD model. ANOVA with Šidák’s post-hoc test was performed. ** P < 0.01 F: ELISA of Aβ40 and Aβ42 secreted into organoid cultivation media. Aβ42/Aβ40 normalised to WT DD60 is shown. Each dot represents an individual organoid (n = 10–12; 3 independent experiments) and the lines represent mean ± SEM. ANOVA with Šidák’s post-hoc test was performed. * P < 0.05; ** P < 0.01; *** P < 0.001

Article Snippet: List of primary and secondary antibodies is as follows: Aβ (Cell Signaling, 8243), SOX2 (Cell Signaling, 3579), PAX6 (Cell Signaling, 60433), NeuN (Millipore, MAB377), CTIP2 (Cell Signaling, 12120), MAP2 (Millipore, AB5543), TUJ (Aves Labs, TUJ-0020), Donkey Anti-Chicken AF647 (Jackson ImmunoResearch, 703-606-155), Donkey Anti-Mouse AF488 (Thermo Fisher Scientific, A-21202), Donkey Anti-Rabbit AF568 (Thermo Fisher Scientific, Cat# A-10042), and Donkey Anti-Rabbit AF647 (Thermo Fisher Scientific, A-31573).

Techniques: Immunofluorescence, Expressing, Generated, Enzyme-linked Immunosorbent Assay